{"product_id":"abcam-ab252780","title":"Abcam, ab252780, Anti-XBP1 antibody [143F] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nMouse Recombinant Monoclonal XBP1 antibody. Carrier free. Suitable for WB, ICC\/IF, IHC-P and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:143F,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody specifically recognizes XBP1-s but not XBP1-u.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab252780 is the carrier-free version of\u003cbr\u003e\nab241571\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXBP1 also known as X-box binding protein 1 is a transcription factor with a mass of approximately 33 kDa. It is mainly expressed in the endoplasmic reticulum of cells where it plays a significant role in regulating the unfolded protein response (UPR). XBP1 exists in two forms: an unspliced form (XBP1u) and a spliced form (XBP1s). The splicing of XBP1 mRNA is mediated by the endoribonuclease IRE1 and is a response to endoplasmic reticulum stress. This process results in a shift of the reading frame which leads to the production of a new functional transcription factor.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe action of XBP1 participates in processes critical for cell survival under stress conditions particularly in the UPR. It activates genes involved in protein folding secretion and degradation to alleviate stress within the endoplasmic reticulum. XBP1 acts as part of the larger mechanism that ensures cellular homeostasis by maintaining protein quality control. It forms a complex network with other transcription factors ensuring an integrated response to stress stimuli in various tissues including pancreatic beta cells and immune cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nXBP1 functions intricately within the UPR and is linked to the insulin signaling pathway. It works alongside proteins such as ATF6 and PERK to coordinate the cellular response to endoplasmic reticulum stress and sustain essential cellular processes. The IRE1-XBP1 pathway represents one arm of the UPR and shows interaction with C\/EBP homologous protein (CHOP) as part of the stress signaling pathways modulating apoptosis if adaptation fails. XBP1 also influences lipid metabolism and cellular differentiation through its pathway connections impacting wide-ranging physiological processes.\u003cbr\u003e\nDysfunctions of XBP1 have associations with metabolic diseases and multiple myeloma. Altered XBP1 activity links to diabetes due to its role in protein homeostasis in pancreatic beta cells involving modulation of insulin secretion and sensitivity. In cancer particularly multiple myeloma XBP1 interacts with proteins such as B-cell maturation antigen (BCMA) influencing cell survival and proliferation. Targeting the XBP1 signaling axis shows potential as a therapeutic strategy for managing disorders resulting from dysregulated protein folding and stress responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855375814825,"sku":"ab252780","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab252780","provider":"Iright","version":"1.0","type":"link"}