{"product_id":"abcam-ab252890","title":"Abcam, ab252890, Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nPhosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue, Suspension cells, Adherent cells,\u003cbr\u003e\nSensitivity:= 50 mU\/well,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD , which can be easily detected (OD= 340 nm). The assay is simple, sensitive and can detect Phosphoglycerate Kinase Activity lower than 50 mU in variety of samples.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K194 Phosphoglycerate Kinase Activity Assay Kit (Colorimetric). K194-100 is the same size as the 100 test size of ab252890.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.\u003cbr\u003e\nPGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843639660713,"sku":"ab252890","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab252890","provider":"Iright","version":"1.0","type":"link"}