{"product_id":"abcam-ab253561","title":"Abcam, ab253561, Human C3\/C3b Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nHuman C3\/C3b Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human C3\/C3b.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:19.53 - 2500 pg\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman C3\/C3b Antibody Pair is a matched pair of unconjugated\u003cbr\u003e\nrecombinant rabbit monoclonal\u003cbr\u003e\ncapture and detection antibodies used to quantify Human C3\/C3b in sandwich ELISAs and many other pair-based applications.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nC3 also known as complement C3 or anti C3b is a central protein in the complement system with an approximate molecular mass of 187 kDa. This protein exists in blood plasma as an inactive precursor and is produced mainly in the liver. C3 gets activated through cleavage into two fragments C3a and C3b. The functional fragment C3b covalently attaches to pathogen surfaces through a process called opsonization allowing it to 'tag' pathogens for destruction by immune cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nComplement C3b plays an integral role in innate immunity. C3b is a part of the complement cascade forming complexes such as C3bBb which acts as a convertase in the amplification of the complement response. Through opsonization C3b promotes the binding and uptake of pathogens by phagocytes therefore enhancing immune defense.\u003cbr\u003e\nPathways\u003cbr\u003e\nC3b integrates into the alternative and classical complement pathways. In these pathways C3b binds with factors like B and D contributing to the formation of the C5 convertase and progressing to the membrane attack complex. These interactions involve proteins such as C5 and Factor B essential for the effective destruction of pathogens and the clearance of immune complexes.\u003cbr\u003e\nComplement C3b shows associations with diseases involving improper immune regulation such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). Mutations affecting C3b's interactions with regulatory proteins such as Factor H can lead to uncontrolled complement activation which contributes to the pathology of these diseases. Understanding these interactions provides insights into therapeutic targets for managing complement-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843605680297,"sku":"ab253561","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab253561","provider":"Iright","version":"1.0","type":"link"}