{"product_id":"abcam-ab255730","title":"Abcam, ab255730, Rat IL-1 beta ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat IL-1 beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-1 beta with a sensitivity of 26.58 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 60 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Citrate plasma, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 26.58 pg\/mL,\u003cbr\u003e\nRange:54.69 - 3500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRat IL-1 beta ELISA Kit ab255730 is a rapid single-wash 90-min Sandwich ELISA to measure Rat IL-1 beta in cell culture media, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 26.58 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nRat IL-1 beta SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nRat IL-1 beta ELISA Kit ab255730 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-IL-1 beta antibody [EPR16805-15] - BSA and Azide free (Capture)\u003cbr\u003e\nab244864\u003cbr\u003e\n- Anti-IL-1 beta antibody [EPR19779-124] - BSA and Azide free (Detector)\u003cbr\u003e\nab244864\u003cbr\u003e\nInterleukin-1 beta (IL-1 beta) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1 beta is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1 beta plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843543978153,"sku":"ab255730","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab255730","provider":"Iright","version":"1.0","type":"link"}