{"product_id":"abcam-ab255745","title":"Abcam, ab255745, Anti-Proinsulin antibody [3B6-19-1] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nMouse Recombinant Monoclonal Insulin antibody. Carrier free. Suitable for Dot, IHC-P, Flow Cyt (Intra) and reacts with Recombinant full length protein, Human, Rat, Mouse samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:3B6-19-1,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), IHC-P, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab255745 is the carrier-free version of\u003cbr\u003e\nab243141\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProinsulin is a precursor molecule important for insulin production. Its alternate names include 'INS-IGF2' or 'preproinsulin'. It has a molecular mass of approximately 9 kDa. The primary expression occurs in the beta cells of the pancreatic islets. Proinsulin undergoes enzymatic cleavage to produce insulin and C-peptide executing a significant transformation as it matures into active insulin which plays an important role in glucose metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProinsulin facilitates the synthesis and release of insulin an essential process involving the conversion from proinsulin to insulin. Proinsulin itself does not operate as part of a complex but requires processing enzymes like prohormone convertase to mature into functional insulin. The efficient conversion of proinsulin to insulin allows cells to maintain glucose homeostasis highlighting its importance in cellular energy usage.\u003cbr\u003e\nPathways\u003cbr\u003e\nInsulin biosynthesis and secretion and the glucose homeostasis pathway rely heavily on proinsulin. In the insulin biosynthesis pathway proinsulin acts upstream of insulin the hormone directly regulating blood glucose levels. The pathways involve interaction with proteins such as proprotein convertase subtilisin\/kexin type 1 (PCSK1) which participates in the conversion of proinsulin to insulin. Through these pathways proinsulin ensures proper energy regulation and balances the body's glucose supply with cellular demands.\u003cbr\u003e\nAberrations in proinsulin processing or levels closely associate with diabetes mellitus and hyperinsulinemia. Diabetes mellitus characterized by insulin deficiency or resistance can result from insufficient proinsulin conversion to insulin. Hyperinsulinemia involves excess levels of insulin-related peptides sometimes due to incomplete proinsulin processing. Disorders affecting proinsulin often involve its relationship with insulin where inadequate or excess production directly influences blood glucose regulation and disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854649512105,"sku":"ab255745","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab255745","provider":"Iright","version":"1.0","type":"link"}