{"product_id":"abcam-ab255960","title":"Abcam, ab255960, Anti-SERCA2 ATPase antibody [IID8] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nMouse Recombinant Monoclonal SERCA2 ATPase antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:IID8,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This antibody does not discriminate between the two isoforms.By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization.This antibody is not recommended for Western blot detection of rat SERCA2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab255960 is the carrier-free version of\u003cbr\u003e\nab2817\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSERCA2 ATPase also known as sarco\/endoplasmic reticulum Ca²⁺-ATPase 2 or ATP2A2 is an important pump responsible for the active transport of calcium ions from the cytosol into the sarcoplasmic or endoplasmic reticulum. This protein has a molecular weight of 110 kDa. It plays an important role in maintaining calcium homeostasis in muscle cells and is expressed in various tissues but particularly in cardiac and skeletal muscle. This pump uses energy from ATP hydrolysis to move calcium ions against their concentration gradient.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe proper regulation of calcium ion levels by SERCA2 ATPase is essential for muscle contraction and relaxation cycles. SERCA2 exists as a part of the complex machinery in muscle cells closely interacting with phospholamban in the cardiac muscle to regulate its activity. Efficient functioning of SERCA2 directly influences calcium ion storage in the sarcoplasmic reticulum affecting muscle physiology and performance.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of SERCA2 ATPase in the calcium signaling pathway and excitation-contraction coupling is significant. The protein's function is intimately linked with RYR2 (ryanodine receptor 2) as they both participate in the regulation of calcium ion flow in muscle cells. This interaction is necessary for the proper release and uptake of calcium ions during muscle contraction and relaxation cycles.\u003cbr\u003e\nSERCA2 ATPase mutations or dysregulation can lead to cardiac diseases such as heart failure and muscle disorders like Brody myopathy. Heart failure often results from impaired calcium handling due to disruptions in the interaction between SERCA2 and associated proteins like phospholamban. Abnormalities in SERCA2 expression or function can lead to either excessive storage or insufficient release of calcium contributing to the pathological state of these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854639419561,"sku":"ab255960","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab255960","provider":"Iright","version":"1.0","type":"link"}