{"product_id":"abcam-ab255985","title":"Abcam, ab255985, Anti-cleaved C-terminal GSDMD antibody [EPR20859-147] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal GSDMD antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR20859-147,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab255985 is the carrier-free version of\u003cbr\u003e\nab255603\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCleaved C-terminal GSDMD also known as cleaved gasdermin D is a protein involved in cellular processes influencing cell death. It is a fragment derived from gasdermin D (GSDMD) after cleavage which is facilitated by inflammatory caspases. This cleavage removes an inhibitory domain allowing the active N-terminal to initiate its functions. The molecular weight of GSDMD is approximately 53 kDa. Cleaved GSDMD is often detected through methods like Western blot particularly in immune cells such as macrophages and monocytes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCleaved C-terminal GSDMD contributes significantly to the process of pyroptosis a form of programmed cell death that is inflammation-driven. This fragmented form is important for forming pores in the cell membrane leading to cellular lysis and the release of pro-inflammatory signals. Gasdermin D including its cleaved form is not known to be part of a large complex on its own but acts autonomously after activation by other proteins like caspase-1.\u003cbr\u003e\nPathways\u003cbr\u003e\nCleaved C-terminal GSDMD participates actively in the inflammatory response particularly in the inflammasome pathway. The protein is closely linked to caspases like caspase-1 that activate it leading to pyroptotic cell death. This pathway also intersects with other innate immune components such as the NLRP3 inflammasome to magnify inflammatory signals and recruit additional immune cells to sites of infection or damage.\u003cbr\u003e\nCleaved C-terminal GSDMD plays a role in conditions featuring excessive inflammation like septicemia and inflammatory bowel disease (IBD). In these diseases aberrant activation and overproduction of cleaved GSDMD can magnify tissue damage through uncontrolled cell death and inflammation. Also in septicemia GSDMD interacts with proteins like caspase-11 which can exacerbate inflammatory cytokine release and worsen the disease course.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851736666281,"sku":"ab255985","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab255985","provider":"Iright","version":"1.0","type":"link"}