{"product_id":"abcam-ab256390","title":"Abcam, ab256390, Mouse MSP ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse MSP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MSP with a sensitivity of 129.22 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 129.22 pg\/mL,\u003cbr\u003e\nRange:312.5 - 20000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse MSP ELISA Kit (ab256390) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MSP protein in cit plasma, edta plasma, hep plasma, serum, urine, tissue lysate, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MSP with 129.22 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nMacrophage stimulating protein (MSP) is a serum protein also known as hepatocyte growth factor-like that is encoded by the *MST1* gene. Secreted by the liver as the main source, MSP has the ability to exert biological effects such as proliferation, migration, differentiation, and phagocytosis through binding to the receptor tyrosine kinase. The standard protein in this kit is a fragment of the full length protein that contains the alpha chain.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMacrophage Stimulating Protein (MSP) also known as hepatocyte growth factor-like protein is a cytokine that plays a role in immune response. MSP is a polypeptide composed of 711 amino acids and has a mass of approximately 80 kDa. This protein is mainly expressed in the liver and circulates in the blood as an inactive precursor. When it gets cleaved by serine proteases it transforms into an active form. The receptor for MSP is the RON receptor tyrosine kinase which mediates most of MSP's functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMSP influences macrophage activation and migration. It is an important factor in inflammation and tissue repair processes. MSP does not commonly exist as a part of larger complexes but its activity is heavily dependent on its interaction with the RON receptor. Through this receptor MSP promotes the anti-inflammatory response and contributes to cell survival and proliferation. It also affects epithelial cell scattering and motility influencing wound healing.\u003cbr\u003e\nPathways\u003cbr\u003e\nMSP is essential in both the PI3K-AKT and RAS-MAPK signaling pathways. These pathways are important for cell survival growth and proliferation. MSP's interaction with the RON receptor leads to the activation of these pathways which further links it with downstream proteins such as AKT1 and MAPK1. The cross-talk between these proteins results in biological effects that relate to cell movement and repair as well as immune modulation.\u003cbr\u003e\nMSP's role becomes evident in cancer and inflammatory disorders. In cancer MSP levels can influence tumor growth and metastasis due to its synergistic effects with proteins like MET another receptor tyrosine kinase. Increased MSP and RON activity can contribute to malignancy by promoting cellular invasion. In inflammatory conditions irregular MSP function can lead to chronic inflammation due to its impact on macrophage behavior interacting with cytokines like IL-6. Understanding MSP's roles in these conditions can help in developing targeted therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843590803625,"sku":"ab256390","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab256390","provider":"Iright","version":"1.0","type":"link"}