{"product_id":"abcam-ab256400","title":"Abcam, ab256400, Mouse ACE ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse ACE ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse ACE with a sensitivity of 24 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 24 pg\/mL,\u003cbr\u003e\nRange:46.88 - 3000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse ACE ELISA Kit (ab256400) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACE protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ACE with 24 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nACE (Angiotensin-converting enzyme) converts angiotensin I to angiotensin II by release of the terminal His-Leu dipeptide, this results in an increase of the vasoconstrictor activity of angiotensin. It is also able to inactivate bradykinin, a potent vasodilator. ACE has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAngiotensin Converting Enzyme 1 (ACE) also called peptidyl-dipeptidase A is a critical enzyme with a mass of approximately 150-180 kDa. It is mainly expressed in the lungs kidneys and endothelial cells. Mechanically ACE converts angiotensin I to angiotensin II by removing a dipeptide. This conversion is significant in the regulation of blood pressure. ACE also degrades bradykinin a peptide that causes blood vessels to dilate which further affects cardiovascular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nACE is deeply involved in cardiovascular homeostasis and fluid balance. As a zinc metalloprotease it acts at the intersection of important physiological processes. It does not operate directly as part of a complex but interacts dynamically with substrates like angiotensin I and bradykinin. ACE activity results in the production of angiotensin II a potent vasoconstrictor which plays a role in blood pressure regulation and has effects on the electrolyte balance in the body.\u003cbr\u003e\nPathways\u003cbr\u003e\nACE holds a central role in the renin-angiotensin system (RAS) and kinin-kallikrein system. In the RAS pathway it transforms angiotensin I into angiotensin II which directly impacts blood pressure regulation through vasoconstriction. Angiotensin II also influences the secretion of aldosterone from the adrenal cortex modulating electrolyte and fluid balance. Additionally ACE modulates the kinin-kallikrein system by degrading bradykinin which influences vasodilation and inflammation.\u003cbr\u003e\nACE has implications in hypertension and heart failure. Dysregulation of ACE activity contributes significantly to these conditions by altering levels of angiotensin II and bradykinin. Angiotensin II's role in vasoconstriction and inflammation ties ACE to the pathology of hypertension. In heart failure altered RAS activity including ACE function can exacerbate disease progression. Related proteins such as angiotensinogen and the angiotensin II type 1 receptor (AT1R) are directly involved in these disorders outlining the interconnected nature of ACE within these pathophysiological contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843582054569,"sku":"ab256400","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab256400","provider":"Iright","version":"1.0","type":"link"}