{"product_id":"abcam-ab256474","title":"Abcam, ab256474, Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Caspase-7 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22840-25,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab256474 is the carrier-free version of\u003cbr\u003e\nab256469\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCleaved Caspase-7 also known as CASP-7 or cleaved Caspase is a protein involved in the execution phase of apoptosis. Its molecular weight is approximately 34 kDa before activation. It belongs to the family of cysteine-aspartic proteases and originates from a precursor with higher molecular weight. Cleavage activates it facilitating its expression in various tissues especially in locations where apoptosis is a regular process such as the liver heart and brain.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCleaved Caspase-7 plays a role by executing apoptosis through cleaving cellular substrates after aspartic acid residues. It operates independently or as part of the apoptosome complex which includes other caspases and protein factors. This complex contributes to the orderly dismantling of cellular structures during programmed cell death ensuring the removal of damaged or unnecessary cells in a controlled manner.\u003cbr\u003e\nPathways\u003cbr\u003e\nCleaved Caspase-7 participates actively in the apoptosis signaling pathway and to a lesser extent in the inflammatory response. It acts downstream in the cascade initiated by pro-apoptotic signals wherein it shares significant functional similarities with Caspase-3. Together with Caspase-8 which acts earlier in the pathway these proteins form a network that ensures the fidelity of programmed cell death. Caspase-8 initiates the cascade leading to the activation of Caspase-7.\u003cbr\u003e\nCleaved Caspase-7 associates notably with cancer and neurodegenerative diseases. Dysregulation of its activity often linked through aberrant apoptosis can lead to the unchecked growth of cancer cells or excessive neuronal death as seen in disorders like Alzheimer's disease. Caspase-8 is often implicated in these pathologies as well highlighting their intertwined roles in cell death and survival pathways. Consequently studying cleaved Caspase-7 helps improve understanding of and develop therapeutic strategies for these significant health issues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848559120553,"sku":"ab256474","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab256474","provider":"Iright","version":"1.0","type":"link"}