{"product_id":"abcam-ab25734","title":"Abcam, ab25734, Anti-CENPB antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-CENPB antibody (ab25734) is a rabbit polyclonal antibody detecting CENPB in  Western Blot, ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - Over 40 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-CENPB antibody (ab25734) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of CENPB?\u003cbr\u003e\nAnti-CENPB (ab25734) specifically detects a band for CENPB (UniProt: P07199) at a molecular weight of 65kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-CENPB (ab25734) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-CENPB (ab25734) has over 5 independent reviews from customers.\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-CENPB antibody (ab25734) has been confirmed by Western blot testing in CENPB Knockout A431 cells.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCENP-B also known as Centromere Protein B is a protein with a molecular weight of approximately 80 kDa. It binds to the centromeric DNA and plays a critical role in centromere formation and function. CENP-B is heavily expressed in dividing cells particularly in tissues where cell proliferation is high such as bone marrow and epithelial tissues. Researchers often use tools like anti-CENP-B antibodies and CENP-B ELISA kits to study its expression in various experimental settings.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCENP-B participates in the assembly of the centromere protein complex contributing to the stability and maintenance of the centromere structure during cell division. It acts as a foundation for kinetochore nucleation ensuring proper chromosome segregation. CENP-B is involved in the formation of the CENP-A nucleosome complex important for accurate chromosomal alignment and separation.\u003cbr\u003e\nPathways\u003cbr\u003e\nCENP-B is essential in the mitotic spindle assembly and chromosome segregation pathways. This protein interacts with CENP-A a variant histone which integrates into centromeric nucleosomes to initiate the formation of functional kinetochores. Additionally CENP-B interacts with CENP-C facilitating the recruitment of other centromere-associated proteins needed for chromosome dynamics during mitosis.\u003cbr\u003e\nCENP-B is primarily associated with autoimmune disorders such as systemic sclerosis (scleroderma). Patients with these conditions often produce anti-CENP-B antibodies which serve as diagnostic markers. Moreover CENP-B's dysregulation links to chromosomal instability a contributor to cancer. Research has shown connections between CENP-B and related proteins like CENP-A in the context of chromosomal aneuploidies and malignancies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844239282345,"sku":"ab25734","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab25734","provider":"Iright","version":"1.0","type":"link"}