{"product_id":"abcam-ab257722","title":"Abcam, ab257722, Human SYNJ1 (Synaptojanin) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSYNJ1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SYNJ1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSynaptojanin also known as SYNJ is a phosphoinositide phosphatase enzyme with a typical molecular mass of around 150 kDa. It functions mechanically by dephosphorylating phosphatidylinositol phosphates at various positions playing an important role in membrane curvature and endocytosis. Its expression is remarkably high in the brain especially in nerve terminals where it assists in synaptic vesicle recycling and neurotransmitter release. Synaptojanin expression also occurs in other tissues but at lower levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSynaptojanin modulates synaptic function and plasticity through its role in lipid metabolism. It is part of a complex with endocytic proteins such as endophilin and dynamin which facilitates the uncoating of clathrin-coated vesicles following endocytosis. This complex action is important for maintaining synaptic efficiency and adaptability especially during high rates of synaptic activity. The disruption of synaptojanin's role affects the proper balance of phospholipids impacting neural communication and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nSynaptojanin plays a significant role in the phosphoinositide signaling and endocytic pathways. It works closely with other proteins like phosphatidylinositol-45-bisphosphate (PIP2) and adaptor protein 2 regulating membrane trafficking and the recycling of synaptic vesicles. These pathways are central to maintaining cellular homeostasis and ensuring neurotransmitter release and recycling occur effectively allowing cells to respond swiftly to dynamic signaling requirements.\u003cbr\u003e\nResearchers link synaptojanin to neurological conditions like Parkinson's disease and Charcot-Marie-Tooth disease. Mutations in synaptojanin or its interaction partners such as endophilin have been shown to disrupt synaptic vesicle reformation leading to neurodegeneration. Studies suggest that variants in synaptojanin can result in synaptic dysfunction contributing to the pathophysiology of these disorders. Understanding synaptojanin's function helps in the development of therapeutic strategies for related neurodegenerative diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847383797929,"sku":"ab257722","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab257722","provider":"Iright","version":"1.0","type":"link"}