{"product_id":"abcam-ab258062","title":"Abcam, ab258062, Human NDUFA11 (NDUAB) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNDUFA11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NDUFA11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNDUAB also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 is an important component of the mitochondrial electron transport chain. This protein has a mass of approximately 14 kDa and plays an essential role in cellular respiration by facilitating the transfer of electrons. It is expressed in the mitochondria across various cell types enabling the production of ATP the energy currency of cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNDUAB is part of a larger complex specifically Complex I also called NADH:ubiquinone oxidoreductase. This complex is the first enzyme of the mitochondrial electron transport chain and is responsible for catalyzing the transfer of electrons from NADH to ubiquinone. NDUAB supports the electron transfer mechanism allowing for the establishment of a proton gradient across the mitochondrial membrane. The proton gradient is vital for ATP synthesis through oxidative phosphorylation.\u003cbr\u003e\nPathways\u003cbr\u003e\nNDUAB's function plays an important role in the oxidative phosphorylation pathway. This pathway is important for the energy metabolism of aerobic organisms and links to the citric acid cycle. NDUAB collaborates with other proteins in Complex I like NDUFS1 and NDUFA10 to facilitate electron transport and proton pumping necessary for ATP production. The proper function of this process is essential for sustaining cellular energy levels.\u003cbr\u003e\nNDUAB has been linked to mitochondrial diseases such as Leigh syndrome and mitochondrial encephalomyopathy. These disorders typically result from dysfunctions in mitochondrial complex I where NDUAB and related proteins such as NDUFS4 and NDUFAF2 may be involved. These diseases often lead to neurological and muscular symptoms due to impaired ATP production highlighting the importance of NDUAB in maintaining cellular energy homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845219635369,"sku":"ab258062","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258062","provider":"Iright","version":"1.0","type":"link"}