{"product_id":"abcam-ab258167","title":"Abcam, ab258167, Human RALBP1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRALBP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RALBP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRALBP1 also known as ralA binding protein 1 or RLIP76 is a multifunctional protein involved in various cellular processes. It has a molecular mass of approximately 76 kDa. RALBP1 often associates with cell membranes and is expressed ubiquitously in different tissues indicating its widespread role in cellular functioning. The protein interacts with small GTPases such as RalA playing critical roles in signal transduction processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRALBP1 serves as an effector protein involved in several cellular activities including endocytosis and transport of glutathione conjugates. It functions as part of a complex with partner proteins facilitating the movement of substrates across cellular membranes. This role is essential for maintaining cellular homeostasis as it helps to eliminate toxic compounds and regulate intracellular signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nRALBP1 participates in the Rho family small GTPase signaling and the Ras signaling pathways. Within these pathways the protein coordinates with RalA and other GTPases to control the trafficking of vesicles and stress response mechanisms. Through these interactions RALBP1 plays an important role in cytoskeletal reorganization and cellular response to extracellular cues.\u003cbr\u003e\nRALBP1 has associations with cancer and metabolic conditions. Alterations in its function or expression levels may contribute to oncogenic processes as RALBP1 interacts with factors like the epidermal growth factor receptor (EGFR) which is often dysregulated in cancerous cells. Moreover its role in detoxification processes implicates RALBP1 in metabolic disorders potentially affecting the body's capability to manage oxidative stress.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845085024425,"sku":"ab258167","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258167","provider":"Iright","version":"1.0","type":"link"}