{"product_id":"abcam-ab258438","title":"Abcam, ab258438, Human GNB1 knockout HCT116 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGNB1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp insertion in exon5 and Insertion of the selection cassette in exon5.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HCT116,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Colon,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp insertion in exon5 and Insertion of the selection cassette in exon5.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GNB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe GNB1 protein also known as Guanine nucleotide-binding protein subunit beta-1 is an important component in signal transduction across a variety of systems. With an approximate mass of 37 kDa GNB1 is expressed in numerous tissues throughout the human body including the central nervous system heart and spleen. Its role is to facilitate communication between receptor proteins on the cell surface and the interior machinery of the cell enabling efficient relay of external signals.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nG-protein signaling inhibitors rely on the interaction of GNB1 within the cellular framework. GNB1 is part of a larger protein complex called heterotrimeric G-proteins. This complex includes three subunits: alpha beta and gamma where GNB1 typically partners with the gamma subunit to stabilize and enhance signal transduction. GNB1 modulates diverse biological processes by enabling proper functioning of neurotransmitter and hormone receptors.\u003cbr\u003e\nPathways\u003cbr\u003e\nGNB1 plays a role in the GPCR (G-protein-coupled receptor) signaling pathway which is one of the largest and most varied signal transduction pathways. Additionally GNB1 influences the MAPK signaling pathway which is important for regulating processes such as cell proliferation and differentiation. In these pathways GNB1 interacts closely with various proteins such as G-alpha subunits and mitogen-activated proteins ensuring precise and targeted signaling outcomes.\u003cbr\u003e\nMutations or dysregulation of GNB1 can lead to significant health issues such as intellectual disability and cancer. GNB1 abnormalities have been linked to an increased susceptibility to neurodevelopmental disorders where its interaction with G-alpha proteins becomes compromised. Additionally irregular GNB1 activity can influence oncogenic pathways contributing to the progression of certain types of cancer by altering cell signaling dynamics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845186572457,"sku":"ab258438","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258438","provider":"Iright","version":"1.0","type":"link"}