{"product_id":"abcam-ab258511","title":"Abcam, ab258511, Human MC2R knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMC2R KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MC2R, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe melanocortin 2 receptor (MC2-R) also known as ACTH receptor is a protein that mechanically functions as a G protein-coupled receptor (GPCR). It is responsible for mediating activity through adenylate cyclase signaling by binding with adrenocorticotropic hormone (ACTH). This receptor has a molecular mass of approximately 33 kDa. MC2-R is mostly expressed in the adrenal cortex where it plays a role in synthesizing and releasing glucocorticoids.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe action of MC2-R initiates the adrenal response to stress through glucocorticoid production which is important for maintaining homeostasis in the body. MC2-R functions as a standalone entity rather than as part of a larger complex. By binding to ACTH MC2-R triggers downstream signaling that leads to steroidogenesis. The biosynthesis of glucocorticoids helps in managing energy immune function and circadian rhythm.\u003cbr\u003e\nPathways\u003cbr\u003e\nMC2-R is integral in the cAMP signaling pathway and the stress response pathway. In the cAMP signaling activation of MC2-R by ACTH results in increased production of cyclic AMP further promoting enzyme activation essential for glucocorticoid synthesis. Its activity is connected to the broader steroid hormone biosynthesis pathway working closely with proteins like StAR (steroidogenic acute regulatory protein) which facilitates cholesterol transport to the inner mitochondrial membrane.\u003cbr\u003e\nDisruptions in MC2-R function can lead to conditions like familial glucocorticoid deficiency and Cushing's syndrome. Dysfunctional receptor signaling may lead to inadequate glucocorticoid production or excessive hormone levels. The role of MC2-R in these disorders connects it closely with proteins like MC1-R another melanocortin receptor involved in pigmentation and inflammatory response which also signifies related signaling cascade abnormalities.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845186277545,"sku":"ab258511","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258511","provider":"Iright","version":"1.0","type":"link"}