{"product_id":"abcam-ab258539","title":"Abcam, ab258539, Human NPM3 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNPM3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 11 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 11 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NPM3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe nucleophosmin\/nucleoplasmin family member 3 (NPM3) also known as nucleoplasmin 3 is a small acidic protein with a molecular mass of approximately 22 kDa. NPM3 localizes mainly in the nucleolus where it participates in various nuclear processes. The protein is expressed widely with higher levels in tissues such as the thymus and testes. It belongs to the histone chaperone family and shares a structural similarity with nucleophosmin (NPM1) promoting its involvement in chromatin assembly and disassembly.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNPM3 plays a role in the regulation of chromatin structure and gene expression. It forms complexes with other proteins to facilitate the proper folding and deposition of histone proteins onto DNA. This process is essential for maintaining the dynamic nature of chromatin allowing genes to be accessed and transcribed as needed. NPM3's function contributes to the efficiency of DNA replication and repair mechanisms within the cell potentially affecting cell cycle progression.\u003cbr\u003e\nPathways\u003cbr\u003e\nNPM3 is involved in key cellular mechanisms such as DNA replication and repair as well as the regulation of transcription. It interacts with proteins involved in the nucleosome assembly pathway including NPM1 which enhances its role in these critical tasks. NPM3's association with these pathways highlights its influence on maintaining genomic integrity and proper cellular function by ensuring accurate DNA duplication and protection from genomic instability.\u003cbr\u003e\nNPM3's dysfunction or altered expression can contribute to the development of cancer particularly hematological malignancies like leukemia. Studies have shown a connection between NPM3 and the oncogenic processes involving NPM1 which is frequently mutated in certain cancer types. Additionally alterations in NPM3 expression have been observed in neural disorders such as Alzheimer's disease implicating its role in neurodegenerative processes possibly through its impact on chromatin dynamics and neuronal gene expression regulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847389008041,"sku":"ab258539","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258539","provider":"Iright","version":"1.0","type":"link"}