{"product_id":"abcam-ab258639","title":"Abcam, ab258639, Human RNASEH2A (Ribonuclease H2, subunit A) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRNASEH2A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RNASEH2A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRibonuclease H2 subunit A also known as RNASEH2A is one part of the RNase H2 enzyme complex. This protein weighs approximately 33 kilodaltons and plays an important role in RNA-DNA hybrid processing. RNASEH2A operates by cleaving the RNA strand of RNA-DNA hybrids which is necessary for maintaining genome stability. The protein expresses in a variety of tissues. Importantly it is found in high levels in cells with rapid division like embryonic and stem cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRNASEH2A facilitates the removal of ribonucleotides from DNA which helps in DNA replication and repair. As a part of the RNase H2 complex alongside subunits B and C it ensures the correct removal of embedded ribonucleotides from DNA strands. This process is important for maintaining the integrity of the genome and preventing mutations. The activity of RNASEH2A directly impacts DNA replication fidelity and overall genome stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nRNASEH2A plays an important role in the maintenance of genome integrity and the DNA repair pathway. It directly interacts with the process of ribonucleotide excision repair which prevents genomic instability. RNASEH2A works alongside enzymes involved in DNA replication and repair mechanisms like DNA polymerases to correct DNA strands. The collaboration of these proteins ensures the accuracy of genomic information passed during cell division.\u003cbr\u003e\nRNASEH2A is associated with Aicardi-Goutières syndrome a severe neurodevelopmental disorder. This syndrome results from mutations in any of the RNase H2 subunits including RNASEH2A leading to accumulated DNA damage and a chronic interferon response. Another condition linked to malfunctions in this protein is systemic lupus erythematosus where an abnormal immune response attacks the body’s own cells. Both conditions connect RNASEH2A with the regulation of immune responses and highlight its role in preventing inappropriate autoimmunity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845134504105,"sku":"ab258639","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258639","provider":"Iright","version":"1.0","type":"link"}