{"product_id":"abcam-ab258723","title":"Abcam, ab258723, Human TMEM97 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM97 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM97, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM97 also known as MAC30 or sigma-2 receptor is a transmembrane protein with a mass of about 23 kDa. This protein is expressed in various tissues with high levels observed in the brain liver and lung. TMEM97 is located in the endoplasmic reticulum and is also present in the plasma membrane suggesting its involvement in multiple cellular processes. Researchers find TMEM97's expression modulated in certain conditions which indicates its potential roles in cellular adaptation and response mechanisms.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM97 interacts with cholesterol influencing its homeostasis within the cells. It plays a role as a receptor that mediates cholesterol regulation and transport. TMEM97 partners with the low-density lipoprotein receptor (LDLR) family to assist in these functions. It is thought to function in complex with other proteins impacting how cells manage cholesterol levels and their own lipid compositions. This gives TMEM97 a significant position in cellular lipid metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM97 participates in cholesterol biosynthesis and transport pathways. It associates with LXR\/RXR signaling which is important for lipid homeostasis and cholesterol metabolism. TMEM97 and its interaction with LDL receptors place it centrally in these pathways influencing how cholesterol is absorbed and processed. TMEM97's involvement suggests a critical role in maintaining lipid balance and supporting healthy cellular functions through these pathways.\u003cbr\u003e\nTMEM97's connection to cholesterol regulation links it to conditions like Alzheimer's disease and cancer growth. Changes in TMEM97 expression or function may result in altered cholesterol homeostasis which is implicated in neurodegenerative diseases like Alzheimer's. In cancer TMEM97 influences tumor cell growth and survival possibly through its regulation of cholesterol pathways. Its relationship with proteins like the LDL receptor in these contexts shows TMEM97's importance in disease progression and potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845129719977,"sku":"ab258723","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258723","provider":"Iright","version":"1.0","type":"link"}