{"product_id":"abcam-ab258735","title":"Abcam, ab258735, Human TRIM16 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTRIM16 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon4.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TRIM16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTRIM16 also known as Tripartite Motif Containing 16 is a protein involved in several cellular mechanisms. It has a molecular mass of approximately 64 kDa. This protein is part of the TRIM protein family characterized by the presence of a RING finger domain one or two zinc-binding motifs called B-boxes and a coiled-coil region. TRIM16 is expressed in many tissues including the skin lungs and brain. The protein exhibits E3 ubiquitin ligase activity which tags other proteins for degradation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein plays a role in regulating cell differentiation and proliferation. TRIM16 is not known to form large complexes but its interactions with other proteins allow it to exert its biological effects. It interacts with factors that influence cellular growth pathways contributing to the control of cellular homeostasis. Its functional roles include modulation of specific transcription factors and involvement in the regulation of inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\nTRIM16 participates in the interferon signaling pathway and the Wnt\/β-catenin signaling pathway. Its involvement in the interferon signaling pathway indicates a role in immune response regulation while its activity in the Wnt\/β-catenin pathway suggests a function in cellular development and differentiation. TRIM16 interacts with proteins like β-catenin and STAT1 integrating signals from different pathways to maintain normal cellular functions.\u003cbr\u003e\nAlterations in TRIM16 expression and function associate with cancer and inflammatory diseases. In cancer TRIM16 often acts as a tumor suppressor with its underexpression linked to increased tumorigenic potential. It interacts with proteins such as p53 to carry out these functions. In the context of inflammatory diseases TRIM16 influences cytokine production closely connected with inflammation regulation and interacts with proteins like NF-kB.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855788527785,"sku":"ab258735","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258735","provider":"Iright","version":"1.0","type":"link"}