{"product_id":"abcam-ab258768","title":"Abcam, ab258768, Human ZMYM3 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nZMYM3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ZMYM3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nZMYM3 also known as Zinc Finger MYM-Type Containing 3 is a protein with a molecular mass of about 155 kDa. It acts as a transcriptional regulator by binding to specific DNA sequences through its zinc-finger domains. ZMYM3 is mainly expressed in the brain and testes suggesting its role in neural and reproductive functions. The protein localizes primarily in the nucleus where it interacts with chromatin to modulate the expression of target genes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThere are multiple roles of ZMYM3 in cellular processes. It is part of the CoREST complex a multiprotein scaffold involved in transcriptional repression. Through its participation in this complex ZMYM3 contributes to chromatin remodeling and epigenetic silencing of genes playing a role in maintaining the balance of gene expression necessary for normal cellular operations and development.\u003cbr\u003e\nPathways\u003cbr\u003e\nThere are several ways ZMYM3 impacts cellular communication and regulation. The protein is involved in the Notch signaling pathway which is important for cell differentiation proliferation and apoptotic processes. ZMYM3 influences this pathway alongside related proteins such as CoREST and LSD1. Additionally it interacts within the androgen receptor signaling pathway potentially impacting gene transcription relevant to sexual development and hormone response.\u003cbr\u003e\nThere is significant interest in the connection between ZMYM3 and cognitive impairments including X-linked intellectual disability. Mutations or dysregulation in ZMYM3 can lead to disrupted transcriptional control linking the protein to neurological disorders. Another medical condition linked to ZMYM3 is prostate cancer where it may affect androgen receptor pathways thereby implicating interactions with proteins like LSD1 and the androgen receptor itself in the disease's progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845190602921,"sku":"ab258768","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258768","provider":"Iright","version":"1.0","type":"link"}