{"product_id":"abcam-ab258841","title":"Abcam, ab258841, Human DDX60L knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nDDX60L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon14 and 4 bp insertion in exon14.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon14 and 4 bp insertion in exon14.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DDX60L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDDX60L also known as DExD\/H-box helicase 60-like is a protein involved in the unwinding of RNA molecules. With a mass of approximately 147 kDa it plays a significant role in RNA metabolism. This protein is mostly expressed in immune-related tissues such as the spleen and lymph nodes. DDX60L through its helicase activity binds and processes RNA which impacts the host antiviral response mechanism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe functions of DDX60L include sensing viral RNA and activating downstream immune responses. It associates with other similar proteins to form a complex that initiates sensing pathways for RNA viruses. This engagement leads to the activation of innate immune responses which are essential for detecting and responding to infections. DDX60L is part of signaling complexes contributing to the regulation and modulation of immune pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nDDX60L plays a pivotal role in the RIG-I-like receptor (RLR) signaling pathway. This pathway detects viral RNAs and initiates antiviral responses through interferon production. DDX60L interacts with proteins like RIG-I (retinoic acid-inducible gene I) to regulate the type I interferon signaling pathway enhancing the body's ability to fight viral infections. The protein contributes to maintaining cellular homeostasis under immune stress conditions.\u003cbr\u003e\nDDX60L is linked to viral infections such as influenza and hepatitis. Its role in the antiviral response makes it a potential target for interventions designed to enhance immune responses against these infections. Additionally the protein's interaction with RIG-I suggests a contributory role in disorders related to dysfunctional immune responses potentially impacting autoimmune conditions where viral components play a part. Understanding DDX60L's function could inform therapeutic approaches to manage these viral-induced disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845186703529,"sku":"ab258841","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258841","provider":"Iright","version":"1.0","type":"link"}