{"product_id":"abcam-ab258860","title":"Abcam, ab258860, Human EML4 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nEML4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 5 bp deletion in exon3 and 95 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 5 bp deletion in exon3 and 95 bp deletion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-EML4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEML4 also known as Echinoderm Microtubule-Associated Protein-Like 4 is a microtubule-associated protein with a mass of approximately 120 kDa. This protein stabilizes microtubules and influences their organization during cell division. EML4 is widely expressed in various tissues particularly in the brain where it plays important roles in neuronal function. It interacts with microtubule structures to ensure proper chromosome segregation and mitotic spindle formation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEML4 facilitates microtubule stability and dynamics which are critical for intracellular transport and cell division. It is part of the EML family and can form complexes with other microtubule-associated proteins. EML4 helps in the regulation of structural arrangements that are important in both mitosis and meiosis processes. Its function in axonal transport becomes essential for proper neuronal development and maintenance.\u003cbr\u003e\nPathways\u003cbr\u003e\nEML4 is involved in the MAPK\/ERK and Ras signaling pathways. Within these pathways EML4 interacts with key proteins like KRAS and BRAF contributing to signal transduction that controls cell proliferation and differentiation. The protein's regulatory role in microtubule dynamics positions it as a link between cellular signaling and cytoskeletal organization.\u003cbr\u003e\nEML4 is most commonly associated with non-small cell lung cancer (NSCLC). A gene fusion between EML4 and ALK (anaplastic lymphoma kinase) leads to the production of an oncogenic protein that drives tumorigenesis. This EML4-ALK fusion protein alters normal cell signaling pathways promoting cancer cell survival and growth. Research also connects EML4 to neurodegenerative diseases due to its important role in microtubule stabilization within neurons.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845170712745,"sku":"ab258860","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258860","provider":"Iright","version":"1.0","type":"link"}