{"product_id":"abcam-ab258871","title":"Abcam, ab258871, Human FGF13 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nFGF13 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-FGF13, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFGF13 also known as Fibroblast Growth Factor 13 is a member of the FGF family and plays a significant role in cellular processes. It has an approximate molecular mass of 29 kDa. You often find FGF13 expressed in the brain and heart indicating its involvement in the physiology of these organs. FGF13 does not function as a traditional growth factor through receptors but instead operates intracellularly engaging directly with cellular components.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFGF13 plays important roles in regulating neuronal development and intracellular signaling pathways. It forms complexes with other proteins modulating their activities within cells. FGF13 is instrumental in neuronal outgrowth and synaptic plasticity essential functions for maintaining a healthy nervous system. Researchers identify FGF13 as important for proper neuronal positioning and survival.\u003cbr\u003e\nPathways\u003cbr\u003e\nFGF13 is intricately involved in signaling mechanisms associated with ion channel regulation and mitogen-activated protein kinase (MAPK) pathway. It interacts with proteins such as kinases and other ion channel components to fine-tune cellular responses to extracellular stimuli. FGF13 by modulating these pathways assists in the precise control of cellular growth differentiation and adaptation.\u003cbr\u003e\nFGF13 presents a significant connection to neurodevelopmental and cardiac disorders. Mutations in FGF13 or its altered expression levels have links to disorders such as epilepsy and cardiac arrhythmia. Through these diseases FGF13 often interacts with proteins like ion channels affecting their function and stability. These interactions highlight the potential of FGF13 as a target for therapeutic interventions in related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845190930601,"sku":"ab258871","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258871","provider":"Iright","version":"1.0","type":"link"}