{"product_id":"abcam-ab258884","title":"Abcam, ab258884, Human GNB4 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGNB4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and 7 bp deletion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and 7 bp deletion in exon 2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GNB4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGNB4 also known as G-protein subunit beta-4 is a component of the heterotrimeric G protein complex which plays a significant role in intracellular signaling. This protein has an approximate molecular weight of 39 kDa. GNB4 is primarily expressed in the brain and also detected in various tissues contributing to its diverse functional roles. As a β-subunit GNB4 forms part of a larger complex that mediates various signal transduction processes by interacting with different receptors on the cell surface.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe functionality of GNB4 involves interactions within the heterotrimeric G protein complex a critical component of signal transduction pathways. This protein acts as a modulator or adapter in transmembrane signaling systems. GNB4 pairs with gamma subunits to help regulate downstream effector proteins expanding the impact of receptor-binding events initiated by external stimuli. GNB4 influences processes like cell communication and neurotransmission making it an important factor in maintaining cellular functions in neural networks.\u003cbr\u003e\nPathways\u003cbr\u003e\nGNB4 plays essential roles in G-protein coupled receptor (GPCR) signaling and other signaling pathways. In GPCR signaling GNB4 works in concert with alpha and gamma subunits to mediate signal propagation. The complex modulates cyclic AMP levels and activates phospholipase C affecting various cellular responses. GNB4 interacts closely with proteins such as GNAS and ADCY1 forming part of the intricate signaling networks that drive cellular communication and response mechanisms.\u003cbr\u003e\nAlterations or dysfunction of GNB4 can relate to certain neuropathological conditions. Mutations in the GNB4 gene have been linked to Charcot-Marie-Tooth disease which leads to peripheral neuropathy. This condition correlates with disruptions in GNB4's role in neuron signaling pathways. Additionally GNB4 interacts with proteins like PMP22 which when mutated also associates with this neuropathic disorder highlighting its involvement in these significant health conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845166354601,"sku":"ab258884","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258884","provider":"Iright","version":"1.0","type":"link"}