{"product_id":"abcam-ab258896","title":"Abcam, ab258896, Human HELZ2 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHELZ2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon9 and 1 bp insertion in exon9.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon9 and 1 bp insertion in exon9.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HELZ2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHELZ2 also known as Helicase Like Transcription Factor 2 is a protein characterized by its capacity to bind DNA and RNA. It possesses a DEAD box domain which makes it part of the DExH\/D box helicases. HELZ2 has a molecular mass of about 247 kDa. This protein is widely expressed in various tissues especially abundant in the liver and brain. Additional research highlights its presence in the adipose tissue indicating its potential role in metabolic processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHELZ2 functions in gene expression regulation by interacting with nuclear receptors and transcription factors. It acts within a protein complex influencing transcriptional regulation through chromatin remodeling. By modulating key transcriptional activities HELZ2 affects numerous genetic pathways and signal transductions contributing to fundamental cellular functions including development and differentiation. Knockout studies of HELZ2 dbd or domain-binding disruption suggest significant involvement in cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis protein plays significant roles in lipid metabolism and the insulin signaling pathway. HELZ2 has connections to related proteins such as PPAR and SREBPs involved in regulating lipid homeostasis and metabolic balance. Through these pathways HELZ2 navigates the intricate regulatory network that maintains metabolic equilibrium exhibiting a profound influence on cellular metabolic processes.\u003cbr\u003e\nHELZ2 is linked to obesity and type 2 diabetes due to its role in metabolic regulation. By interacting with PPAR and other metabolic regulators HELZ2 has implications in adipogenesis and energy homeostasis which are critical in the pathogenesis of these metabolic disorders. Understanding its function may offer insights into potential therapeutic targets for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845183492265,"sku":"ab258896","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258896","provider":"Iright","version":"1.0","type":"link"}