{"product_id":"abcam-ab258940","title":"Abcam, ab258940, Human LAPTM4A knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nLAPTM4A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 7 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 7 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-LAPTM4A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLAPTM4A also known as Lysosomal-associated transmembrane protein 4 alpha is a protein involved in intracellular trafficking. It has a molecular weight of approximately 28 kDa. LAPTM4A consists of four predicted transmembrane domains and expresses in various tissues with high levels in the spleen kidney and liver. It plays a role in endosomal and lysosomal pathways influencing cellular processes like nutrient transport and recycling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein contributes to cell comprehension by facilitating lysosomal function and maintaining cellular homeostasis. LAPTM4A participates in the regulation of proliferation and survival often forming a complex with other lysosomal proteins for transmitting signals related to these processes. It impacts cellular signaling pathways by interacting with certain membrane receptors and transporting them to lysosomes for degradation or recycling.\u003cbr\u003e\nPathways\u003cbr\u003e\nLAPTM4A is an integral part of endocytic and vesicular transport pathways. It influences receptor-mediated endocytosis that directs cell surface proteins to lysosomes for degradation or recycling. In these pathways LAPTM4A interacts with other proteins like c-MET a receptor tyrosine kinase that plays a part in signal transduction processes linked to cell growth.\u003cbr\u003e\nMutations or alterations in LAPTM4A levels can connect to cancer and psychiatric disorders like schizophrenia. The protein's interaction with c-MET has identified its role in tumor progression where abnormal c-MET signaling contributes to cancerous growth. Furthermore alterations in LAPTM4A can affect neurotransmitter systems associating the protein with schizophrenia's pathophysiology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847385829545,"sku":"ab258940","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258940","provider":"Iright","version":"1.0","type":"link"}