{"product_id":"abcam-ab258942","title":"Abcam, ab258942, Human LIN7C knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nLIN7C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 26 bp deletion in exon1 and 7 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 26 bp deletion in exon1 and 7 bp insertion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-LIN7C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLIN7C also known as Lin-7 homolog C or Veli3 is a protein associated with the regulation of cellular processes. This protein weighs approximately 24 kDa and is expressed in various tissues including the brain and epithelial cells. LIN7C belongs to the LIN-7 family characterized by their PDZ domains which are important for protein-protein interactions. The protein plays a significant role in maintaining cell polarity which is essential for the proper functioning of cells in different tissue types.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLIN7C contributes significantly to cellular signaling and organization. It operates as part of a multiprotein complex alongside DLG1 and CASK interacting with several other proteins to facilitate cell adhesion and synaptic transmission. This assembly orchestrates the anchoring of membrane proteins influencing the spatial distribution of signaling receptors. LIN7C's role ensures that proteins reach and maintain their proper membrane location important for successful synaptic and epithelial cell functioning.\u003cbr\u003e\nPathways\u003cbr\u003e\nLIN7C is actively involved in the PI3K\/AKT pathway critical for cell survival and proliferation. LIN7C assists in stabilizing interactions with signaling molecules like GRIN2B contributing to synaptic plasticity and memory formation. Additionally LIN7C engages with the Wnt signaling pathway which influences cell development and differentiation through interactions with proteins such as LRP6. This interaction emphasizes LIN7C's importance in processes that guide cellular growth and differentiation.\u003cbr\u003e\nLIN7C has connections to neurological conditions such as autism spectrum disorders and schizophrenia. Its involvement in maintaining synaptic stability and neurotransmission links it to these disorders often characterized by synaptic dysfunction. Moreover dysregulation of LIN7C affects interactions with proteins such as DLG4 contributing to the pathophysiology of these conditions. Understanding LIN7C’s role offers insight into potential therapeutic targets for mitigating synaptic-related dysfunctions seen in these neuropsychiatric disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845169565865,"sku":"ab258942","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258942","provider":"Iright","version":"1.0","type":"link"}