{"product_id":"abcam-ab258973","title":"Abcam, ab258973, Human MSL1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMSL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon 3 and 1 bp insertion in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon 3 and 1 bp insertion in exon 3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MSL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMSL1 or Male-Specific Lethal 1 functions mechanically as an important component of the MSL complex that engages in gene regulation activities. It has a molecular mass of approximately 62 kDa. MSL1 expresses in diverse tissues including the brain liver and muscle. It plays a critical role in dosage compensation contributing to equalizing gene expression levels between sexes in species like Drosophila.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMSL1 participates in the assembly of the MSL complex. This multi-protein complex facilitates histone H4 lysine 16 acetylation which activates transcription. MSL1 specifically assists in recruiting the acetyltransferase MOF to target sites on the X chromosome. The expression level and activity of MSL1 are tightly regulated to ensure proper chromosomal balance in the organism. Its activity is not limited to dosage compensation but also influences other chromatin remodeling processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMSL1 plays significant roles in chromatin modification pathways participating in transcriptional regulation actively. The protein aligns closely with MOF in these pathways forming a duo that heavily influences acetylation across chromatin landscapes. In addition to histone modification MSL1 connections extend to gene expression pathways responding to various cellular stimuli interfacing with proteins like MSL2 and MSL3 to regulate gene activation machinery.\u003cbr\u003e\nMSL1 has associations with certain cancers including breast cancer and leukemia. The protein's dysregulation can skew chromatin remodeling leading to abnormal gene expression profiles commonly seen in tumorigenesis. MSL1 partners with other proteins like MOF impacting the transcriptional regulatory networks often targeted in cancer therapies. Understanding MSL1's role allows for targeted approaches in developing therapeutic strategies for these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845187489961,"sku":"ab258973","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258973","provider":"Iright","version":"1.0","type":"link"}