{"product_id":"abcam-ab258983","title":"Abcam, ab258983, Human NABP2 (hSSB1) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNABP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NABP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHSSB1 also known as OBFC2B is a single-stranded DNA-binding protein with a molecular mass of approximately 30 kDa. It is mainly expressed in the nucleus where it plays an important role in the maintenance and protection of genomic stability. The protein recognizes and binds to single-stranded DNA which is typical during DNA replication and repair processes. hSSB1 is highly conserved across different species highlighting its important function in cellular mechanisms.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein plays a significant role in DNA damage response and repair. hSSB1 forms a complex with the INTS3 protein as part of the MRN (MRE11-RAD50-NBS1) complex to effectively respond to DNA double-strand breaks. This action is critical to initiate the appropriate signaling cascade that leads to DNA repair. The protein's expression and activity need tight regulation to ensure genome integrity and to prevent mutations.\u003cbr\u003e\nPathways\u003cbr\u003e\nHSSB1 is involved in the homologous recombination repair pathway which is essential for error-free repair of DNA double-strand breaks. It interacts with BRCA1 and RAD51 which are key players in homologous recombination ensuring accurate DNA damage repair. Additionally hSSB1 participates in the ATR signaling pathway enhancing cell cycle checkpoint activation during replication stress where it collaborates with ATR and its substrates.\u003cbr\u003e\nHSSB1 has been implicated in cancer development particularly in breast and ovarian cancers. Defects or dysregulation in hSSB1 may lead to inadequate DNA repair increasing the likelihood of mutations that contribute to carcinogenesis. Furthermore hSSB1 has connections to neurodegenerative disorders linked through its interactions with proteins like ATM. hSSB1's influence on maintaining genomic stability places it at the intersection of various pathways that affect cellular health and disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845219930281,"sku":"ab258983","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258983","provider":"Iright","version":"1.0","type":"link"}