{"product_id":"abcam-ab258985","title":"Abcam, ab258985, Human NADK knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNADK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NADK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNAD kinase also known as NADK is a critical enzyme responsible for phosphorylating nicotinamide adenine dinucleotide (NAD+) to form NADP+. NADK has an approximate molecular weight of 50 kDa. Its expression is widespread but it is especially abundant in pancreatic and liver tissues. This enzyme converts NAD+ to NADP+ which is important for maintaining the balance of NADP+\/NADPH within cells impacting several biochemical processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe conversion role played by NADK is essential for maintaining cellular redox status and for biosynthetic reactions that require NADPH such as fatty acid synthesis and detoxifying reactive oxygen species. NADK functions independently without being part of larger protein complexes. It facilitates key cellular processes by ensuring adequate levels of NADPH a necessary cofactor in various anabolic reactions and cellular defense mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nNADK prominently features in the pentose phosphate pathway which is important for the production of NADPH. It interacts with enzymes like glucose-6-phosphate dehydrogenase (G6PD) which is also involved in producing NADPH through this pathway. Additionally NADK plays a role in the citric acid cycle where its activity supports energy metabolism by maintaining cellular NADP+\/NADPH ratios.\u003cbr\u003e\nAltered NADK activity links to conditions such as cancer and diabetes. Its role in regulating redox state and metabolic pathways means that dysregulation can lead to oxidative stress contributing to the pathogenesis of several types of cancer. Disrupted glucose metabolism in diabetes associates G6PD and NADK since both are pivotal in maintaining NADPH levels highlighting the enzyme's connection with metabolic diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845213769897,"sku":"ab258985","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab258985","provider":"Iright","version":"1.0","type":"link"}