{"product_id":"abcam-ab259000","title":"Abcam, ab259000, Human NPTN knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nNPTN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 5 bp deletion in exon3 and 8 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 5 bp deletion in exon3 and 8 bp deletion in exon3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NPTN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNeuroplastin (NPTN) also known as Basigin-like protein 1 plays an important role in various cellular functions. This protein has a mass of approximately 34 kDa as determined by its amino acid sequence. NPTN locates itself on the cell membrane and exhibits expression in multiple tissues especially in the nervous system and skeletal muscles. This protein is known for participating actively in cell adhesion processes which influence neural development and synaptic plasticity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNeuroplastin contributes to important cellular processes like calcium homeostasis and synapse formation. As part of the oligomeric receptor complex it facilitates calcium-mediated signaling by forming functional partnerships with other membrane proteins such as plasma membrane Ca2+ ATPases. Neuroplastin's interactions influence dendritic spine morphology and synaptic strength which are essential for proper neuronal communication and memory formation.\u003cbr\u003e\nPathways\u003cbr\u003e\nNeuroplastin functions significantly in the Calcium signaling pathway which regulates numerous cellular activities including muscle contraction and neurotransmitter release. It associates with proteins like PMCA playing a role in calcium ion efflux and maintaining intracellular calcium concentration. Additionally Neuroplastin is involved in the MAP kinase signaling pathway which contributes to cell viability and adaptation to stress.\u003cbr\u003e\nNeuroplastin shows linkage to conditions such as schizophrenia and hearing loss. Abnormal expression or mutations of NPTN can disrupt neural connectivity and synaptic functions potentially leading to psychiatric disorders. Furthermore studies associate NPTN with Basigin in disorders affecting auditory capacities suggesting possible therapeutic targets for intervention in inner ear function deficits.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845182443689,"sku":"ab259000","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab259000","provider":"Iright","version":"1.0","type":"link"}