{"product_id":"abcam-ab259011","title":"Abcam, ab259011, Human OAS1 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nOAS1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-OAS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe OAS1 protein also known as 2'-5'-oligoadenylate synthetase 1 plays an important role in the antiviral response of the body. It has a molecular mass of approximately 42 kDa. This protein is expressed in various tissues including the liver lungs and heart. OAS1 is part of a family of interferon-induced enzymes that synthesize 2'-5'-linked oligoadenylates which are important for cellular defense mechanisms against viral infections.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe creation of 2'-5'-linked oligoadenylates by OAS1 is essential in activating RNase L an enzyme that degrades viral and cellular RNA to impede viral replication. OAS1 does not operate as part of a larger protein complex but functions independently to mediate antiviral activities. It has been observed that upon viral infection the levels of OAS1 increase enhancing the interferon response and cytokine signaling.\u003cbr\u003e\nPathways\u003cbr\u003e\nOAS1 plays a significant role in the interferon signaling pathway and innate immune response pathways. Within these pathways OAS1 interacts with other proteins such as RNase L and is also associated with STAT1 a critical mediator of the cellular response to interferons. The function of OAS1 within these pathways highlights its importance in modulating immune responses to pathogens.\u003cbr\u003e\nOAS1 has been linked to conditions such as viral infections and autoimmune diseases. Its role in viral infections involves the detection and degradation of viral RNA thereby limiting viral proliferation. The interaction of OAS1 with RNase L is important in this process. In autoimmune disorders certain variants of OAS1 can alter immune function potentially contributing to abnormal immune responses and disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845114548393,"sku":"ab259011","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab259011","provider":"Iright","version":"1.0","type":"link"}