{"product_id":"abcam-ab259143","title":"Abcam, ab259143, Human SMARCD1 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSMARCD1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 2 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 2 bp insertion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SMARCD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSMARCD1 also known as SWI\/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a component of the SWI\/SNF chromatin remodeling complex. The protein has a mass of approximately 50 kDa. It shows expression in various tissues including the brain liver and heart. Alternative names for SMARCD1 include BAF60A indicating its role within the BAF (BRG1\/BRM-associated factors) complex.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSMARCD1 plays a significant role in altering chromatin structure to regulate gene expression. It functions as part of the SWI\/SNF complex which modulates access of transcription factors to DNA. This protein contributes to the regulation of various genes involved in cellular processes like proliferation differentiation and DNA repair. The SWI\/SNF complex including SMARCD1 alters nucleosome positioning affecting the transcriptional landscape.\u003cbr\u003e\nPathways\u003cbr\u003e\nSMARCD1 influences the expression of genes involved in critical cellular activities. It participates actively in the Wnt signaling and the cell cycle regulation pathways. The Wnt pathway includes critical interactions with proteins like β-catenin which contributes to the regulation of gene transcription related to cell fate and proliferation. SMARCD1's involvement in cell cycle regulation also implicates interactions with proteins such as cyclins and p53.\u003cbr\u003e\nThe altered expression or mutation of SMARCD1 relates to conditions like cancer and developmental disorders. Overexpression of SMARCD1 has associations with certain types of cancers including breast cancer where SMARCD1 modulates interactions with key proteins like BRG1 and BRM. Developmental disorders may involve interactions affecting the SWI\/SNF complex leading to issues in tissue differentiation and growth.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845086924969,"sku":"ab259143","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab259143","provider":"Iright","version":"1.0","type":"link"}