{"product_id":"abcam-ab259167","title":"Abcam, ab259167, Human STX17 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSTX17 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and 4 bp deletion in exon4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon4 and 4 bp deletion in exon4.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-STX17, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSTX17 also known as Syntaxin 17 is a SNARE protein involved in membrane fusion processes. Its molecular weight is approximately 34 kDa. STX17 is predominantly expressed in the endoplasmic reticulum and associated membranes. The protein plays a mechanical role in mediating the fusion of vesicles with target membranes facilitating the transport of molecules within cells. STX17 localizes mostly in cell types associated with high metabolic activity where it supports cellular trafficking demands.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSyntaxin 17 contributes to the formation of a multiprotein complex involved in the fusion of autophagosomes with lysosomes. This process is important for macroautophagy where damaged organelles and proteins are degraded and recycled. The protein forms a functional unit with other SNARE proteins including VAMP8 and SNAP29 to carry out its role. It ensures cellular homeostasis by facilitating the clearance of unnecessary or damaged cellular components under stress conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nSyntaxin 17 is critical in the autophagy and endocytosis pathways. These pathways are involved in the degradation and recycling of cellular components maintaining cellular integrity and energy balance. In the autophagy pathway STX17 functions alongside proteins such as LC3 and Beclin-1 coordinating the recognition and processing of autophagosomes. In the endocytosis pathway its interactions help balance the intake and processing of extracellular materials.\u003cbr\u003e\nSTX17 shows significant connections to neurodegenerative disorders and cancer. Its dysfunction in the autophagy process may lead to accumulation of toxic protein aggregates contributing to disorders like Alzheimer's disease. Defects or mutations in the functioning of STX17 have been linked to impaired cellular waste disposal in this context. In cancer alterations in STX17 expression or function can disrupt autophagic processes and affect cancer cell survival. Understanding its link with neurodegenerative proteins such as tau could offer insights for therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845177790633,"sku":"ab259167","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab259167","provider":"Iright","version":"1.0","type":"link"}