{"product_id":"abcam-ab259200","title":"Abcam, ab259200, Human TRAF5 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTRAF5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TRAF5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTRAF5 known as TNF receptor-associated factor 5 plays a role in cellular signaling pathways. This protein has a molecular mass of approximately 64 kDa. It is expressed in various tissues including lymphoid tissues and cells of the immune system. TRAF5 acts in signaling downstream of certain receptors like TNF receptor superfamily where it functions as an adapter protein that mediates signal transduction by interacting with other signaling molecules.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTRAF5 influences immune cell functions and inflammatory responses. It forms part of a larger protein complex that regulates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Through this complex TRAF5 affects the transcription of genes involved in immune responses and cell survival. By modulating these processes TRAF5 helps maintain immune homeostasis and ensures proper immune response.\u003cbr\u003e\nPathways\u003cbr\u003e\nTRAF5 is involved in signaling pathways such as NF-κB and JNK (c-Jun N-terminal kinase) pathways. TRAF5 interacts with proteins like TRAF2 and RIPK1 to propagate signals that lead to activation of these pathways. NF-κB pathway regulates inflammation and immune responses while JNK pathway plays a part in stress responses and apoptosis. The interaction of TRAF5 with these proteins and pathways positions it as an integral part of the immune signaling network.\u003cbr\u003e\nTRAF5 associates with autoimmune diseases including rheumatoid arthritis and lupus. Abnormal signaling through TRAF5 can lead to dysregulated immune responses contributing to these conditions. In rheumatoid arthritis for example TRAF5 may abnormally activate NF-κB pathway leading to enhanced inflammation. Moreover the interaction between TRAF5 and other proteins like TRAF1 influences the development of these diseases by affecting signal transduction which is important for understanding potential therapeutic targets in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855773814953,"sku":"ab259200","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab259200","provider":"Iright","version":"1.0","type":"link"}