{"product_id":"abcam-ab259598","title":"Abcam, ab259598, Anti-DMP1 antibody [EPR22205-14] - BSA and Azide free (Detector)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal DMP1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22205-14,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab259598 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DMP1. The recommended pair for sandwich ELISA is:\u003cbr\u003e\nCapture:\u003cbr\u003e\nab259597\u003cbr\u003e\n, Human DMP1 Capture Antibody (unconjugated)\u003cbr\u003e\nDetector: ab259598, Human DMP1 Detector Antibody (unconjugated)\u003cbr\u003e\nThe reference range value is 171.875 - 11000 pg\/ml.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein DMP1 also known as Dentin Matrix Protein 1 is part of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family and plays an important role in bone and tooth mineralization. It is encoded by the DMP1 gene and has a molecular weight of around 52 kDa. DMP1 localizes mainly to the extracellular matrix of bone and dentin and is highly expressed in osteoblasts odontoblasts and hypertrophic chondrocytes. This protein regulates the formation of hydroxyapatite a critical mineral component in the formation of hard tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein DMP1 is essential for phosphate homeostasis skeletal development and mineralization processes. It acts as a nucleator in the deposition of calcium phosphate minerals. As part of a protein complex DMP1 interacts with other extracellular matrix proteins influencing their functions. DMP1 plays an important role in the maturation of bone matrix and dentin effectively mediating the transformation of mesenchymal stem cells into osteoblasts and odontoblasts. It is also implicated in the regulation of gene expression linked to mineralization.\u003cbr\u003e\nPathways\u003cbr\u003e\nDMP1 is closely involved in the bone mineralization and FGF (Fibroblast Growth Factor) signaling pathways. These pathways are important for maintaining bone density and controlling phosphate metabolism. DMP1 interacts with other proteins like PHEX (Phosphate Regulating Endopeptidase Homolog X-Linked) and MEPE (Matrix Extracellular Phosphoglycoprotein) within these pathways to facilitate proper mineralization of bone and teeth. Furthermore DMP1 functions alongside other SIBLING family proteins to modulate mineralization through their collective roles in the matrix.\u003cbr\u003e\nDMP1 mutations or dysfunctions lead to conditions such as autosomal recessive hypophosphatemic rickets and osteomalacia. These conditions result from defective phosphate metabolism affecting skeletal mineralization and causing brittle bones and dental anomalies. In these scenarios DMP1's relationship with PHEX becomes evident as both proteins work together to regulate phosphate levels. Disruption in their function can significantly alter mineralization balance highlighting the necessity of DMP1 in maintaining skeletal and dental health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848564494505,"sku":"ab259598","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab259598","provider":"Iright","version":"1.0","type":"link"}