{"product_id":"abcam-ab261831","title":"Abcam, ab261831, Human ADRB1 (beta 1 Adrenergic Receptor) knockout HeLa cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 2 x 1000000Cells \/ vial\u003cbr\u003e\nADRB1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ADRB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe beta 1 adrenergic receptor also known as beta-1 adrenoceptor or beta-1 receptor is a G protein-coupled receptor involved in the response to epinephrine and norepinephrine. It has a molecular mass of approximately 51 kDa. This receptor is mainly expressed in the heart where it plays a significant role in regulating cardiovascular functions. It is also found in other tissues like the brain and kidneys though at lower levels. The beta-1 adrenergic receptor functions by activating adenylate cyclase which increases intracellular cAMP levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe beta 1 adrenergic receptor influences heart rate and cardiac contractility. This receptor is key in the sympathetic nervous system affecting physiological responses during stress or exercise. It does not form part of a complex in its active state. Upon activation the receptor enhances cardiac output by increasing the force and frequency of heart contractions. It also contributes to the relaxation of vascular and smooth muscle tissues facilitating better blood flow and pressure regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nBeta 1 adrenergic receptor is integral to the adrenergic signaling pathway. It works alongside proteins like adenylate cyclase to modulate the levels of cAMP within the cell which acts as a secondary messenger in signal transduction. The receptor plays a significant role in the fight-or-flight response coordinating with other adrenergic receptors like the beta 2 adrenergic receptor to ensure a well-rounded physiological reaction to stressors. Another pathway of significance is the cardiac beta-adrenergic signaling which involves the regulation of heart muscle activities.\u003cbr\u003e\nThe beta 1 adrenergic receptor is associated with heart failure and hypertension. In heart failure altered expression or function of this receptor can lead to impaired contractile response and cardiac performance. In hypertension overactive adrenergic receptor signaling contributes to increased cardiac output and blood pressure. Related proteins include the beta-2 adrenergic receptor and angiotensin II which can exacerbate these conditions when dysregulated influencing the development and progression of cardiovascular diseases. Esmolol a beta-1 selective blocker is often used to manage these conditions by targeting the receptor specifically to reduce heart rate and blood pressure.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845139353769,"sku":"ab261831","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab261831","provider":"Iright","version":"1.0","type":"link"}