{"product_id":"abcam-ab263071","title":"Abcam, ab263071, Anti-LINE-1 ORF2p antibody [MT49]","description":"\u003cp\u003eSize: 20µg \/ 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal LINE-1 ORF2p antibody. Suitable for WB, ICC\/IF and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:MT49,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLINE-1 ORF2p also known as L1 ORF2 protein is a critical component in the process of retrotransposition. This protein approximately 150 kDa in mass is encoded by the Long Interspersed Nuclear Element-1 (LINE-1) a type of non-long terminal repeat (non-LTR) retrotransposon. LINE-1 ORF2p contains reverse transcriptase and endonuclease activities which are necessary for inserting copies of LINE-1 DNA back into the genome. This protein is mainly expressed in germ cells and early embryonic tissues although its presence is noted in various somatic tissues in certain contexts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLINE-1 ORF2p facilitates the mobilization and insertion of LINE-1 elements into new genomic locations an activity integral to maintaining genome plasticity. It is not generally classified as part of a larger protein complex but functions closely with another protein LINE-1 ORF1p. ORF2p's reverse transcriptase domain synthesizes DNA from RNA allowing the LINE-1 element to integrate into the host genome. This action implicates it in genomic rearrangements that can have profound effects on gene expression and genome stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nLINE-1 ORF2p plays a substantial role in genomic and epigenomic pathways particularly those involving DNA synthesis and repair. It interacts with cellular proteins such as DNA repair machinery that modulate its insertion capacity and activity. This protein influences the development and function of cellular pathways through its impact on genomic architecture affecting neighboring gene regulation. The interaction with proteins like the Apurinic\/apyrimidinic endonuclease (APE1) is significant as they can regulate LINE-1 insertion events.\u003cbr\u003e\nLINE-1 ORF2p is associated with cancer and neurodegenerative diseases due to its role in genomic instability. Uncontrolled activity of LINE-1 can lead to mutagenic insertions in oncogenes or tumor suppressor genes contributing to tumorigenesis. Additionally in neurodegenerative diseases inappropriate activation of LINE-1 ORF2p may induce neuronal damage through genomic disarray. Related proteins include PIWI-interacting RNAs (piRNAs) which usually silence LINE-1 activity to prevent genomic chaos.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850423128233,"sku":"ab263071","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263071","provider":"Iright","version":"1.0","type":"link"}