{"product_id":"abcam-ab263117","title":"Abcam, ab263117, Human C11orf31 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSELENOH KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SELENOH, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSELH also known as Selenoprotein H is a selenocysteine-containing protein with a molecular mass of approximately 15 kDa. It functions in redox regulation and protection against oxidative stress by facilitating antioxidant defense mechanisms. SELH is mainly expressed in the brain and to a lesser extent in other tissues. It belongs to the selenoprotein family which includes proteins containing the rare amino acid selenocysteine responsible for its unique enzymatic capabilities.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSelenoprotein H contributes to protecting cells from oxidative damage. It plays a role in regenerating antioxidant molecules and maintaining cellular redox balance. SELH is not known to be part of a larger protein complex but works in concert with other antioxidant molecules to enhance cellular resistance to oxidative stress. It also involves gene regulation particularly through modulating transcription factors that respond to oxidative signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nSelenoprotein H engages significantly in the cellular antioxidant pathways and redox homeostasis. It interacts with enzymes like glutathione peroxidase and thioredoxin reductases which are essential in countering oxidative stress. These interactions help maintain signal transduction pathways which depend on redox-sensitive components affecting cellular metabolism and survival. SELH along with these proteins ensures proper cellular function by modulating oxidative status and impacting pathways related to energy metabolism and apoptosis.\u003cbr\u003e\nSelenoprotein H relates notably to neurodegenerative diseases and certain cancers. SELH’s role in protecting neurons from oxidative stress connects it to conditions like Alzheimer's disease where oxidative stress contributes to neuronal degradation. Additionally it is involved with the tumor suppressor protein p53 which responds to oxidative stress in cancer development. SELH’s antioxidant activity can influence these pathways impacting disease progression and therapeutic responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855783841961,"sku":"ab263117","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263117","provider":"Iright","version":"1.0","type":"link"}