{"product_id":"abcam-ab263212","title":"Abcam, ab263212, Human GANC knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGANC KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 4 bp deletion in exon11.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 4 bp deletion in exon11.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GANC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe GANC gene encodes the enzyme Glucosidase Alpha Neutral C (GAN Glucosidase) also commonly known as neutral alpha-glucosidase C. It plays an important role in carbohydrate metabolism by hydrolyzing terminal non-reducing (14)-linked alpha-glucose residues to release alpha-glucose. The enzyme mass is approximately 106 kDa. GANC is widely expressed in human tissues with significant presence in the liver pancreas and intestine where it contributes to glucose homeostasis and nutrient absorption.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis enzyme is important for carbohydrate digestion and glucose production. GANC is not known to form part of a larger protein complex but acts individually to catalyze the breakdown of polysaccharides and oligosaccharides. By converting these molecules into glucose it provides an immediate source of energy for cells thereby supporting cellular metabolism and overall organismal energy balance.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe enzyme takes part in the carbohydrate metabolism pathway and the glycogen degradation pathway. These pathways are instrumental in energy production and storage management in cells. GANC works in conjunction with enzymes like glycogen phosphorylase and beta-amylase to regulate the conversion of stored glycogen into glucose ensuring proper energy supply during fasting and physical activity.\u003cbr\u003e\nGANC associations include diabetes mellitus and glycogen storage disorders. Dysfunction or imbalance in GANC activity can lead to improper glucose regulation characteristic in diabetes where glucose metabolism is impaired. The enzyme collaborates with insulin and glucagon regulation mechanisms to maintain glucose homeostasis. In glycogen storage disorders improper degradation of glycogen can exacerbate energy supply issues highlighting the significance of GANC in maintaining normal glucose levels and metabolic functions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847389860009,"sku":"ab263212","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263212","provider":"Iright","version":"1.0","type":"link"}