{"product_id":"abcam-ab263214","title":"Abcam, ab263214, Human GDE1 (MIR16) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nGDE1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GDE1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMIR16 also known as microRNA-16 is a small non-coding RNA molecule typically around 22 nucleotides in length. This microRNA engages in the post-transcriptional regulation of gene expression impacting various mRNAs by binding and either degrading them or inhibiting their translation. MIR16 is expressed in numerous human tissues including the lungs heart and spleen indicating its broad functional significance in the body.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMIR16 regulates cell cycle progression and apoptosis playing a role in maintaining cellular homeostasis. It is part of the microRNA-induced silencing complex (RISC) enabling it to mediate gene silencing efficiently. By influencing the expression of genes involved in the cell cycle MIR16 helps control cell proliferation and programmed cell death which are important processes for normal cellular function and response to stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nMIR16 is involved in the cell cycle and apoptosis signaling pathways. It interacts with key proteins such as cyclin D1 and Bcl-2 which are essential for cell cycle regulation and apoptosis respectively. By modulating these pathways MIR16 contributes to maintaining the balance between cell survival and death which is necessary to prevent abnormal cell growth.\u003cbr\u003e\nMIR16 has connections to cancer and cardiovascular diseases. Aberrant expression of MIR16 can lead to uncontrolled cell proliferation linking it to cancer progression particularly in chronic lymphocytic leukemia where it shows reduced expression. Moreover its role in regulating apoptosis implicates MIR16 in cardiovascular diseases where its dysregulation can impact heart function. The interaction with proteins such as Bcl-2 in these conditions highlights its potential as a therapeutic target in disease management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847384518825,"sku":"ab263214","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263214","provider":"Iright","version":"1.0","type":"link"}