{"product_id":"abcam-ab263242","title":"Abcam, ab263242, Human KLHDC3 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nKLHDC3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-KLHDC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nKLHDC3 also known as Kelch Domain Containing 3 is a protein with a mass of approximately 30 kDa. It is characterized by the presence of a Kelch domain which enables protein-protein interactions. It is expressed in various tissues with notable presence in the brain and testes. This protein is part of the Kelch domain-containing family which often plays roles in scaffolding and organizing protein complexes within cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nKLHDC3 participates in cellular processes such as transcriptional regulation and cell cycle control. It is involved in forming protein complexes that modify transcriptional responses. These complexes interact with a variety of transcription factors impacting gene expression. KLHDC3 also regulates the stability and degradation of certain proteins by acting as an adapter for ubiquitination processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nKLHDC3 plays a role in critical pathways like the ubiquitin-proteasome system and the Wnt signaling pathway. It interacts with proteins involved in these pathways such as Beta-catenin influencing cellular growth and development. The ubiquitin-proteasome system is essential for protein turnover while Wnt signaling regulates gene transcription that affects embryogenesis and cell proliferation.\u003cbr\u003e\nKLHDC3 has been linked to disorders like neurodegenerative diseases and certain cancers. Aberrant functioning of KLHDC3 or its related pathways can disrupt normal cellular processes leading to disease development. For example its interaction with Beta-catenin can influence pathways associated with tumor progression. Further research aims to understand how KLHDC3 modulations contribute to disease pathology and may guide therapeutic approaches.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855778336937,"sku":"ab263242","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263242","provider":"Iright","version":"1.0","type":"link"}