{"product_id":"abcam-ab263297","title":"Abcam, ab263297, Human PIP4K2C (PIP5K2 gamma) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPIP4K2C KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 334 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 334 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PIP4K2C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPIP5K2 gamma also known as phosphatidylinositol-5-phosphate 4-kinase type-2 gamma is an enzyme encoded by the PIP4K2C gene. It plays an important role in cellular processes by phosphorylating phosphatidylinositol-5-phosphate to generate phosphatidylinositol-45-bisphosphate. The molecular mass of PIP5K2 gamma is approximately 51 kDa. This protein is mostly expressed in brain tissue but researchers have also found it in other tissues including the pancreas and spleen.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein's primary role involves the regulation of phosphoinositide signaling pathways. PIP5K2 gamma participates in membrane trafficking cytoskeletal organization and signal transduction processes. It associates with different protein complexes that modulate these cellular activities. By controlling the levels of phosphatidylinositol-45-bisphosphate PIP5K2 gamma indirectly regulates several downstream signaling events.\u003cbr\u003e\nPathways\u003cbr\u003e\nScientists have shown that PIP5K2 gamma is a part of the phosphatidylinositol signaling system which is important for signal transduction and cellular communication. It interacts closely with proteins such as phospholipase C and AKT. PIP5K2 gamma also contributes to the insulin signaling pathway impacting glucose metabolism and cell proliferation.\u003cbr\u003e\nStudies link PIP5K2 gamma to several medical conditions. Its dysregulation associates with neurological disorders including bipolar disorder. Variations in this protein are also connected to cancer. For example in cancer alterations in the PIP5K2 gamma activity may affect pathways involving the AKT protein leading to uncontrolled cell growth and survival.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855776567465,"sku":"ab263297","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263297","provider":"Iright","version":"1.0","type":"link"}