{"product_id":"abcam-ab263302","title":"Abcam, ab263302, Human PLEKHJ1 (GNRPX) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPLEKHJ1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PLEKHJ1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGNRPX also known as Gonaderexin Protein X plays a significant mechanical role in cellular processes. It has a molecular mass of approximately 45 kDa. This protein shows expression in the gonadal tissues including the testes and ovaries and can be detected in lower levels elsewhere in the body. GNRPX acts as a regulator within the cellular signaling systems pertinent to reproductive functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGNRPX facilitates communication between cellular systems involved in reproductive biology. It acts as a component of a larger protein complex that participates in the regulation of gonadotropin-releasing activities. These processes are essential for normal reproductive function including the regulation of hormone release. GNRPX does not act independently but within a collaboration of proteins that support the reproductive axis.\u003cbr\u003e\nPathways\u003cbr\u003e\nGNRPX is linked with the hypothalamic-pituitary-gonadal (HPG) axis pathway vital for reproductive hormone signaling. Within this axis GNRPX interacts closely with the luteinizing hormone-releasing hormone (LHRH). It maintains important links to ensure the cascade of events that mediate the release of hormones like luteinizing hormone and follicle-stimulating hormone. Disruptions in these interactions can impact hormonal balance and reproductive health.\u003cbr\u003e\nGNRPX has associations with conditions related to reproductive health such as hypogonadotropic hypogonadism. Altered expression or mutations in GNRPX might correlate with disrupted hormone regulation in this disorder. It shows connections to other proteins involved in the disorder pathway like LHRH highlighting its integral role in maintaining hormonal homeostasis. Understanding its involvement in these disorders could aid the development of targeted therapies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845191782569,"sku":"ab263302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263302","provider":"Iright","version":"1.0","type":"link"}