{"product_id":"abcam-ab263329","title":"Abcam, ab263329, Human RNF10 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRNF10 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RNF10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRNF10 also known as RING finger protein 10 is an E3 ubiquitin-protein ligase that plays a role in attaching ubiquitin molecules to target substrates marking them for proteasomal degradation. RNF10 has a molecular weight of approximately 75 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. RNF10 contains a RING finger domain important for its ligase activity allowing it to interact with E2 ubiquitin-conjugating enzymes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRNF10 participates in regulating cellular processes like neurodevelopment and muscle differentiation. It interacts with other proteins to form complexes that influence cell signaling pathways and transcription regulation. This protein's activities affect neuronal growth and synaptic plasticity important for learning and memory. RNF10 also has implications in muscle cells where it modulates the expression of genes related to muscle function.\u003cbr\u003e\nPathways\u003cbr\u003e\nRNF10 takes part in the MAPK signaling pathway which plays a role in cell proliferation and differentiation. In this pathway RNF10 interacts with proteins such as MEK and ERK allowing it to influence downstream signaling processes. Additionally RNF10 has connections to the Wnt signaling pathway impacting gene expression patterns important for development. These interactions highlight its role in orchestrating complex cellular responses.\u003cbr\u003e\nRNF10 is linked to neurological and muscular conditions. Its involvement in neurodevelopment suggests a connection to neurodegenerative diseases like Alzheimer's where protein degradation processes may go awry. In muscular disorders RNF10 may interact with proteins like MYOD1 influencing muscle differentiation and regeneration. These associations highlight the importance of understanding RNF10's role in healthy and diseased states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845189783721,"sku":"ab263329","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263329","provider":"Iright","version":"1.0","type":"link"}