{"product_id":"abcam-ab263341","title":"Abcam, ab263341, Human SDHAF2 (C11orf79) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSDHAF2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SDHAF2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nC11orf79 also known as HAPSTR1 is a protein coding gene. It has a molecular mass of approximately 19 kDa. Researchers have detected its expression in various tissues including the bone marrow spleen and thymus suggesting its physiological role in immune-related contexts. C11orf79 acts mechanistically in cellular contexts where it may influence protein synthesis or transport although detailed mechanical functions remain under investigation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nC11orf79 impacts cellular processes in the immune system and has links to the HAPSTR complex where it interacts with other proteins to regulate hematopoiesis. The protein contributes to maintaining normal cell function by aiding in the regulation of proliferation and differentiation of myeloid and lymphoid cells. This suggests its involvement in general immune response and hematopoietic homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nC11orf79 participates in pathways related to immune cell development and function. Notably it engages in the Notch signaling pathway which influences hematopoietic cell differentiation and lymphocyte development. Additionally it interacts with proteins such as NOTCH1 and RBPJ which are essential in both the regulation of developmental processes and maintaining cellular identity.\u003cbr\u003e\nC11orf79 has associations with immune-related diseases like autoimmune lymphoproliferative syndrome (ALPS) and certain leukemias. Its dysfunction may disrupt normal immune cell regulation exacerbating these conditions. Additionally this gene has interactions with proteins like FAS and BAG5 which can influence cell apoptosis and survival suggesting a role in disease pathogenesis where apoptotic pathways become impaired.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845183197353,"sku":"ab263341","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263341","provider":"Iright","version":"1.0","type":"link"}