{"product_id":"abcam-ab263376","title":"Abcam, ab263376, Human SURF6 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSURF6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon1 and 7 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon1 and 7 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SURF6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSurf6 also known as SURF-6 or Surfeit locus protein 6 is a protein that acts mechanically within the cell nucleus. It is approximately 37 kDa in size and shows a nuclear localization. Surf6 expression takes place in a variety of cell types where it is often located in the nucleolus suggesting its role in ribosomal functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nWithin the nucleolus Surf6 associates with rRNA processing and ribosome biogenesis. It serves as a nucleolar protein with a function in facilitating the maturation and assembly of pre-rRNA into functional ribosomal subunits. Surf6 is not known to form part of a larger complex but shows interactions with other nucleolar components implying a regulatory role in ribosome production.\u003cbr\u003e\nPathways\u003cbr\u003e\nElements involving Surf6 participate in the ribosome biogenesis pathway and the cell cycle regulation pathway. Surf6 has a documented association with the RNA polymerase I machinery which is important for rRNA transcription. Its function aligns with nucleolar proteins like nucleolin and fibrillarin which also regulate ribosomal RNA synthesis and processing.\u003cbr\u003e\nAlterations in Surf6 expression or mutation can link to proliferative diseases such as cancer. Surf6's role in ribosome biogenesis ties it to tumors where excessive ribosome production supports uncontrolled cell division. Connections are evident with other proteins like p53 known for tumor suppressor activities highlighting Surf6's potential impact on cellular proliferation in oncogenic contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845179855017,"sku":"ab263376","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263376","provider":"Iright","version":"1.0","type":"link"}