{"product_id":"abcam-ab263382","title":"Abcam, ab263382, Human TDP2 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTDP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TDP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTDP2 also known as Tyrosyl-DNA phosphodiesterase 2 functions mechanically by repairing DNA through removing topoisomerase II-DNA adducts. It has a mass of approximately 53 kDa. TDP2 shows expression in various tissues with notable levels in brain and muscle. The enzyme exhibits phosphodiesterase activity which is essential for resolving stalled topoisomerase-2-mediated breaks during the repair process.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTDP2 plays an important role in DNA repair mechanisms. It participates in excising 5'-phosphotyrosyl moieties linked to DNAn important for maintaining genomic stability. TDP2 does not work as a part of a large protein complex but its activity supports several cellular processes by ensuring proper DNA repair. It facilitates the release of covalent protein-DNA complexes therefore aiding cell survival and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nTDP2 contributes to the DNA damage response and repair pathways. It acts in concert with proteins like XRCC1 and PARP1 to ensure repair of DNA strand breaks. Additionally TDP2 is involved in the non-homologous end joining (NHEJ) pathway an important mechanism for repairing double-strand breaks. Its function complements topoisomerase-related activities maintaining genomic integrity during cell division.\u003cbr\u003e\nTDP2 has been connected to neurodegenerative diseases and certain cancers. Mutations or dysfunctions in TDP2 can lead to spinocerebellar ataxia and intellectual disability as the enzyme’s role in DNA repair is compromised. In cancer disruptions in TDP2 activity can influence cancer progression with interactions involving proteins like ATM playing a role in oncogenesis. Understanding these relationships highlights potential therapeutic targets for treatment strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845174579369,"sku":"ab263382","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263382","provider":"Iright","version":"1.0","type":"link"}