{"product_id":"abcam-ab263390","title":"Abcam, ab263390, Human TMEM208 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM208 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 41 bp insertion in exon1 and 7 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 41 bp insertion in exon1 and 7 bp deletion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM208, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM208 also known as transmembrane protein 208 plays a role in cellular processes involving the endoplasmic reticulum and lysosomes. With an estimated molecular weight of around 24 kDa scientist can find TMEM208 predominantly expressed in human tissues particularly in the heart and skeletal muscle. As a multi-pass membrane protein TMEM208 establishes the cell organelles' communication ensuring intracellular stability and proper functioning of cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein serves an essential function in the formation and maintenance of autophagic vesicles. It operates as a component of the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) participating in cargo sorting and transport. TMEM208's role in vesicle nucleation is important for autophagy a process where cells remove unnecessary or dysfunctional components maintaining cellular homeostasis. This action indirectly supports cellular adaptation to stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM208 integrates into intracellular trafficking and autophagy pathways working alongside proteins such as LC3 and ATG-related proteins. Through its involvement in vesicular transport TMEM208 collaborates in the lysosomal degradation pathway a process important for recycling cellular materials. TMEM208's interaction with LC3 connects it to the macroautophagy pathway allowing the cell to respond effectively to nutrient deprivation or other cellular stressors.\u003cbr\u003e\nTMEM208 associates with pathologies linked to improper autophagic processes and cellular stress responses including neurodegenerative diseases like Alzheimer's and possibly myopathies. Researchers note its association with proteins such as p62 which accumulates when autophagy is disrupted indicating TMEM208's potential role in disease progression. Understanding TMEM208's function might lead to identifying therapeutic targets for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845172711593,"sku":"ab263390","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263390","provider":"Iright","version":"1.0","type":"link"}